sam levels Search Results


sam 940 defender sodium iodide detector  (Berkeley Nucleonics Corporation)
92
Berkeley Nucleonics Corporation sam 940 defender sodium iodide detector
Sam 940 Defender Sodium Iodide Detector, supplied by Berkeley Nucleonics Corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lc10a hplc system  (Shimadzu Corporation)
90
Shimadzu Corporation lc10a hplc system
Lc10a Hplc System, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transcript-level analysis  (Bambu Vault LLC)
90
Bambu Vault LLC transcript-level analysis
Transcript Level Analysis, supplied by Bambu Vault LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sam levels  (BMG Labtech)
99
BMG Labtech sam levels
Sam Levels, supplied by BMG Labtech, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sam 5630 glycine sodium  (Evonik)
90
Evonik sam 5630 glycine sodium
Sam 5630 Glycine Sodium, supplied by Evonik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s-adenosylmethionine elisa  (Cell Biolabs Inc)
90
Cell Biolabs Inc s-adenosylmethionine elisa
(A) <t>ELISA-measured</t> <t>SAM</t> levels in P18 Pkd1F/RC-KO and P10 Pkd1F/F-KO kidneys compared to their respective age-matched control kidneys.
S Adenosylmethionine Elisa, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adomet  (Thermo Fisher)
90
Thermo Fisher adomet
The new SUMO E1 CAIs perturb polyamine synthesis through the MAT2A-AdoMetDC axis. (a) MAT2A is a SUMOylation substrate and converts methionine (Met) to S-adenosylmethionine <t>(AdoMet).</t> AdoMetDC decarboxylates AdoMet to produce dcAdoMet for the synthesis of spermidine (Spd) and spermine (Spm). ODC decarboxylates ornithine (Orn) to generate putrescine (Put). (b) <t>The</t> <t>cellular</t> level of MAT2A was assessed with Western blotting after T47D cells were treated with the CAIs. (c) The cellular level of AdoMet was assessed with LC-MS after T47D cells were treated with the CAIs. (d) The cellular levels of polyamines were assessed with LC-MS after T47D cells were treated with the CAIs. (e) The cellular levels of two rate-limiting polyamine synthesis enzymes, ODC and AdoMetDC, were assessed with Western blotting after T47D cells were treated with the CAIs.
Adomet, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cs-t300 automatic biochemistry analyzer  (DIRUI Industrial Co Ltd)
90
DIRUI Industrial Co Ltd cs-t300 automatic biochemistry analyzer
The new SUMO E1 CAIs perturb polyamine synthesis through the MAT2A-AdoMetDC axis. (a) MAT2A is a SUMOylation substrate and converts methionine (Met) to S-adenosylmethionine <t>(AdoMet).</t> AdoMetDC decarboxylates AdoMet to produce dcAdoMet for the synthesis of spermidine (Spd) and spermine (Spm). ODC decarboxylates ornithine (Orn) to generate putrescine (Put). (b) <t>The</t> <t>cellular</t> level of MAT2A was assessed with Western blotting after T47D cells were treated with the CAIs. (c) The cellular level of AdoMet was assessed with LC-MS after T47D cells were treated with the CAIs. (d) The cellular levels of polyamines were assessed with LC-MS after T47D cells were treated with the CAIs. (e) The cellular levels of two rate-limiting polyamine synthesis enzymes, ODC and AdoMetDC, were assessed with Western blotting after T47D cells were treated with the CAIs.
Cs T300 Automatic Biochemistry Analyzer, supplied by DIRUI Industrial Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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uplc system  (Waters Corporation)
86
Waters Corporation uplc system
The new SUMO E1 CAIs perturb polyamine synthesis through the MAT2A-AdoMetDC axis. (a) MAT2A is a SUMOylation substrate and converts methionine (Met) to S-adenosylmethionine <t>(AdoMet).</t> AdoMetDC decarboxylates AdoMet to produce dcAdoMet for the synthesis of spermidine (Spd) and spermine (Spm). ODC decarboxylates ornithine (Orn) to generate putrescine (Put). (b) <t>The</t> <t>cellular</t> level of MAT2A was assessed with Western blotting after T47D cells were treated with the CAIs. (c) The cellular level of AdoMet was assessed with LC-MS after T47D cells were treated with the CAIs. (d) The cellular levels of polyamines were assessed with LC-MS after T47D cells were treated with the CAIs. (e) The cellular levels of two rate-limiting polyamine synthesis enzymes, ODC and AdoMetDC, were assessed with Western blotting after T47D cells were treated with the CAIs.
Uplc System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scanning auger microprobe (sam, model 918-2707/2601)  (Varian Medical)
90
Varian Medical scanning auger microprobe (sam, model 918-2707/2601)
The new SUMO E1 CAIs perturb polyamine synthesis through the MAT2A-AdoMetDC axis. (a) MAT2A is a SUMOylation substrate and converts methionine (Met) to S-adenosylmethionine <t>(AdoMet).</t> AdoMetDC decarboxylates AdoMet to produce dcAdoMet for the synthesis of spermidine (Spd) and spermine (Spm). ODC decarboxylates ornithine (Orn) to generate putrescine (Put). (b) <t>The</t> <t>cellular</t> level of MAT2A was assessed with Western blotting after T47D cells were treated with the CAIs. (c) The cellular level of AdoMet was assessed with LC-MS after T47D cells were treated with the CAIs. (d) The cellular levels of polyamines were assessed with LC-MS after T47D cells were treated with the CAIs. (e) The cellular levels of two rate-limiting polyamine synthesis enzymes, ODC and AdoMetDC, were assessed with Western blotting after T47D cells were treated with the CAIs.
Scanning Auger Microprobe (Sam, Model 918 2707/2601), supplied by Varian Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aminomethanesulfonic aul capacity  (Evonik)
90
Evonik aminomethanesulfonic aul capacity
The new SUMO E1 CAIs perturb polyamine synthesis through the MAT2A-AdoMetDC axis. (a) MAT2A is a SUMOylation substrate and converts methionine (Met) to S-adenosylmethionine <t>(AdoMet).</t> AdoMetDC decarboxylates AdoMet to produce dcAdoMet for the synthesis of spermidine (Spd) and spermine (Spm). ODC decarboxylates ornithine (Orn) to generate putrescine (Put). (b) <t>The</t> <t>cellular</t> level of MAT2A was assessed with Western blotting after T47D cells were treated with the CAIs. (c) The cellular level of AdoMet was assessed with LC-MS after T47D cells were treated with the CAIs. (d) The cellular levels of polyamines were assessed with LC-MS after T47D cells were treated with the CAIs. (e) The cellular levels of two rate-limiting polyamine synthesis enzymes, ODC and AdoMetDC, were assessed with Western blotting after T47D cells were treated with the CAIs.
Aminomethanesulfonic Aul Capacity, supplied by Evonik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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colistin sulfomethate sodium  (Sam Chun Dang Pharm)
90
Sam Chun Dang Pharm colistin sulfomethate sodium
The new SUMO E1 CAIs perturb polyamine synthesis through the MAT2A-AdoMetDC axis. (a) MAT2A is a SUMOylation substrate and converts methionine (Met) to S-adenosylmethionine <t>(AdoMet).</t> AdoMetDC decarboxylates AdoMet to produce dcAdoMet for the synthesis of spermidine (Spd) and spermine (Spm). ODC decarboxylates ornithine (Orn) to generate putrescine (Put). (b) <t>The</t> <t>cellular</t> level of MAT2A was assessed with Western blotting after T47D cells were treated with the CAIs. (c) The cellular level of AdoMet was assessed with LC-MS after T47D cells were treated with the CAIs. (d) The cellular levels of polyamines were assessed with LC-MS after T47D cells were treated with the CAIs. (e) The cellular levels of two rate-limiting polyamine synthesis enzymes, ODC and AdoMetDC, were assessed with Western blotting after T47D cells were treated with the CAIs.
Colistin Sulfomethate Sodium, supplied by Sam Chun Dang Pharm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) ELISA-measured SAM levels in P18 Pkd1F/RC-KO and P10 Pkd1F/F-KO kidneys compared to their respective age-matched control kidneys.

Journal: Cell metabolism

Article Title: A methionine-Mettl3- N 6 -methyladenosine axis promotes polycystic kidney disease

doi: 10.1016/j.cmet.2021.03.024

Figure Lengend Snippet: (A) ELISA-measured SAM levels in P18 Pkd1F/RC-KO and P10 Pkd1F/F-KO kidneys compared to their respective age-matched control kidneys.

Article Snippet: S-adenosylmethionine ELISA SAM levels in kidney tissues of age-matched control and ADPKD mice were measured using the ELISA kit from Cell Biolabs (# STA-671-C) following the manufacturer's protocol.

Techniques: Enzyme-linked Immunosorbent Assay

KEY RESOURCES TABLE

Journal: Cell metabolism

Article Title: A methionine-Mettl3- N 6 -methyladenosine axis promotes polycystic kidney disease

doi: 10.1016/j.cmet.2021.03.024

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: S-adenosylmethionine ELISA SAM levels in kidney tissues of age-matched control and ADPKD mice were measured using the ELISA kit from Cell Biolabs (# STA-671-C) following the manufacturer's protocol.

Techniques: Plasmid Preparation, Recombinant, Enzyme-linked Immunosorbent Assay, Expressing, Software

The new SUMO E1 CAIs perturb polyamine synthesis through the MAT2A-AdoMetDC axis. (a) MAT2A is a SUMOylation substrate and converts methionine (Met) to S-adenosylmethionine (AdoMet). AdoMetDC decarboxylates AdoMet to produce dcAdoMet for the synthesis of spermidine (Spd) and spermine (Spm). ODC decarboxylates ornithine (Orn) to generate putrescine (Put). (b) The cellular level of MAT2A was assessed with Western blotting after T47D cells were treated with the CAIs. (c) The cellular level of AdoMet was assessed with LC-MS after T47D cells were treated with the CAIs. (d) The cellular levels of polyamines were assessed with LC-MS after T47D cells were treated with the CAIs. (e) The cellular levels of two rate-limiting polyamine synthesis enzymes, ODC and AdoMetDC, were assessed with Western blotting after T47D cells were treated with the CAIs.

Journal: bioRxiv

Article Title: SUMO E1 covalent allosteric inhibitors modulate polyamine synthesis via the MAT2A-AdoMetDC axis

doi: 10.1101/2024.12.12.627095

Figure Lengend Snippet: The new SUMO E1 CAIs perturb polyamine synthesis through the MAT2A-AdoMetDC axis. (a) MAT2A is a SUMOylation substrate and converts methionine (Met) to S-adenosylmethionine (AdoMet). AdoMetDC decarboxylates AdoMet to produce dcAdoMet for the synthesis of spermidine (Spd) and spermine (Spm). ODC decarboxylates ornithine (Orn) to generate putrescine (Put). (b) The cellular level of MAT2A was assessed with Western blotting after T47D cells were treated with the CAIs. (c) The cellular level of AdoMet was assessed with LC-MS after T47D cells were treated with the CAIs. (d) The cellular levels of polyamines were assessed with LC-MS after T47D cells were treated with the CAIs. (e) The cellular levels of two rate-limiting polyamine synthesis enzymes, ODC and AdoMetDC, were assessed with Western blotting after T47D cells were treated with the CAIs.

Article Snippet: The cellular levels of AdoMet and polyamines were quantified on a QE Q Exactive UHMR Hybrid Quadrupole-Orbitrap MS System (Thermo Fisher).

Techniques: Western Blot, Liquid Chromatography with Mass Spectroscopy